p-probabilistic k-anonymous microaggregation for the anonymization of surveys with uncertain participation

We develop a probabilistic variant of k-anonymous microaggregation which we term p-probabilistic resorting to a statistical model of respondent participation in order to aggregate quasi-identifiers in such a manner that k-anonymity is concordantly enforced with a parametric probabilistic guarantee. Succinctly owing the possibility that some respondents may not finally participate, sufficiently larger cells are created striving to satisfy k-anonymity with probability at least p. The microaggregation function is designed before the respondents submit their confidential data. More precisely, a specification of the function is sent to them which they may verify and apply to their quasi-identifying demographic variables prior to submitting the microaggregated data along with the confidential attributes to an authorized repository. We propose a number of metrics to assess the performance of our probabilistic approach in terms of anonymity and distortion which we proceed to investigate theoretically in depth and empirically with synthetic and standardized data. We stress that in addition to constituting a functional extension of traditional microaggregation, thereby broadening its applicability to the anonymization of statistical databases in a wide variety of contexts, the relaxation of trust assumptions is arguably expected to have a considerable impact on user acceptance and ultimately on data utility through mere availability.Peer ReviewedPostprint (author's final draft

Amorphous solid dispersions of curcumin in a poly(ester amide): antiplasticizing effect on the glass transition and macromolecular relaxation dynamics, and controlled release

In order to exploit the pharmacological potential of natural bioactive molecules with low water solubility, such as curcumin, it is necessary to develop formulations, such as amorphous polymer dispersions, which allow a constant release rate and at the same time avoid possible toxicity effects of the crystalline form of the molecule under scrutiny. In this study, polymer dispersions of curcumin were obtained in PADAS, a biodegradable semicrystalline copolymer based on 1,12-dodecanediol, sebacic acid and alanine. The dispersions were fully characterized by means of differential scanning calorimetry and broadband dielectric spectroscopy, and the drug release profile was measured in a simulated body fluid. Amorphous homogeneous binary dispersions were obtained for curcumin mass fraction between 30 and 50%. Curcumin has significantly higher glass transition temperature Tg (˜ 347 K) than the polymer matrix (˜274-277 K depending on the molecular weight), and dispersions displayed Tg’s intermediate between those of the pure amorphous components, implying that curcumin acts as an effective antiplasticizer for PADAS. Dielectric spectroscopy was employed to assess the relaxation dynamics of the binary dispersion with 30 wt% curcumin, as well as that of each (amorphous) component separately. The binary dispersion was characterized by a single structural relaxation, a single Johari-Goldstein process, and two local intramolecular processes, one for each component. Interestingly, the latter processes scaled with the Tg of the sample, indicating that they are viscosity-sensitive. In addition, both the pristine polymer and the dispersion exhibited an interfacial Maxwell-Wagner relaxation, likely due to spatial heterogeneities associated with phase disproportionation in this polymer. The release of curcumin from the dispersion in a simulated body fluid followed a Fickian diffusion profile, and 51% of the initial curcumin content was released in 48 h.Peer ReviewedPostprint (published version

Poly(butylene succinate) matrices obtained by thermally-induced phase separation: Pore shape and orientation affect drug release

Poly(butylene succinate) (PBS) scaffolds with 3D microporous interconnected architecture and crystallized PBS substructures have been prepared by thermally-induced phase separation (TIPS). Curcumin (CUR) and piperine (PIP) natural drugs were incorporated into the matrices during a one-step fabrication protocol. Alterations in TIPS parameters such as solvent system (1,4 dioxane (DXN) or THF) and cooling condition (intensity and direction of thermal gradient) affected the phase separation process and the scaffold properties. Controlling the crystallization of DXN in a uniaxial direction, resulted in the formation of microtubular scaffolds with oriented porosities. Well-developed PIP crystals and matrix-integrated CUR agglomerates were uniformly distributed throughout the scaffolds. The integration of CUR to the polymer matrix was confirmed by physicochemical evaluations, attributed to a possible interaction with PBS, resulting in a slower release than PIP. The scaffolds with uniaxially-oriented porosities displayed a greater sustained release (only 50–60% after 170 h) due to the restricted drug diffusion through their dense spherulitic pore walls. Due to such structure and smoother well-arranged surfaces, the oriented scaffolds also exhibited greater biocompatibility. Our results reveal the positive influence of TIPS-derived structural orientation on properties of PBS matrices for cell/drug delivery.Peer ReviewedPostprint (published version

p-probabilistic k-anonymous microaggregation for the anonymization of surveys with uncertain participation

We develop a probabilistic variant of k-anonymous microaggregation which we term p-probabilistic resorting to a statistical model of respondent participation in order to aggregate quasi-identifiers in such a manner that k-anonymity is concordantly enforced with a parametric probabilistic guarantee. Succinctly owing the possibility that some respondents may not finally participate, sufficiently larger cells are created striving to satisfy k-anonymity with probability at least p. The microaggregation function is designed before the respondents submit their confidential data. More precisely, a specification of the function is sent to them which they may verify and apply to their quasi-identifying demographic variables prior to submitting the microaggregated data along with the confidential attributes to an authorized repository. We propose a number of metrics to assess the performance of our probabilistic approach in terms of anonymity and distortion which we proceed to investigate theoretically in depth and empirically with synthetic and standardized data. We stress that in addition to constituting a functional extension of traditional microaggregation, thereby broadening its applicability to the anonymization of statistical databases in a wide variety of contexts, the relaxation of trust assumptions is arguably expected to have a considerable impact on user acceptance and ultimately on data utility through mere availability.Peer Reviewe

Antimicrobial activity of poly(ester urea) electrospun fibers loaded with bacteriophages

The capacity to load bacteriophages into electrospun nanofibers of two representative biocompatible polymers has been evaluated, paying special attention to the possibility of preserving their antibacterial activity. Specifically, the work involves the following steps: (a) Evaluation of the effect of the applied electrical field on the phage activity; (b) evaluation of the activity when a lyophilization process could be avoided by using water soluble polymers (e.g., poly(ethylene glycol); (c) evaluation of the activity when dissolution of the polymer requires an organic solvent and lyophilization is theoretically necessary. In this case, a poly(ester urea) (PEU) derived from the natural L-leucine amino acid has been considered. Adsorption of commercial bacteriophage preparations into calcium carbonate particles was demonstrated to be a promising methodology to avoid lyophilization and keep the initial bactericide activity at a maximum. Phagestaph and Fersis bacteriophage commercial preparations have been selected for this study due to their spPeer Reviewe